Boykyo Cell-Free DNA BCT 218992 alternative products of Streck

Streck Cell-Free DNA BCT 218961 alternative products of Streck

Boykyo Cell-Free DNA BCT 218992 alternative products of Streck

6-Tube Pack (10 mL) 218961
100-Tube Box (10 mL) 218962
1000-Tube Box (10 mL) 218992
6-Tube Pack (2 mL) 230242
100-Tube Box (2 mL) 230243
Cell-Free DNA BCT CE is For Export Only. Not for sale in the U.S.
6-Tube Pack (10 mL) 218996
100-Tube Box (10 mL) 218997
1000-Tube Box (10 mL) 230244
6-Tube Pack (2 mL) 230250
100-Tube Box (2 mL) 230251

Cell-Free DNA BCT Blood Collection Tube

Boykyo Cell-Free DNA BCT 218992 alternative products of Streck

Streck Cell-Free DNA BCT 218962 10 mL  alternative products of Streck

Now available in two sizes: 10 mL and 2 mL

Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Cell-Free DNA BCT is for Research Use Only. Not for use in diagnostic procedures in the U.S.

Cell-Free DNA BCT CE is for Export Only. Not for sale in the U.S.

Paper: Stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping

Click here to see patents that may be applicable to this product.

Resources
Catalog Numbers
Cell-Free DNA BCT Catalog Number
Cell-Free DNA BCT is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack (10 mL) 218961
100-Tube Box (10 mL) 218962
1000-Tube Box (10 mL) 218992
6-Tube Pack (2 mL) 230242
100-Tube Box (2 mL) 230243
Cell-Free DNA BCT CE is For Export Only. Not for sale in the U.S.
6-Tube Pack (10 mL) 218996
100-Tube Box (10 mL) 218997
1000-Tube Box (10 mL) 230244
6-Tube Pack (2 mL) 230250
100-Tube Box (2 mL) 230251

We are one company from china. Our Company name is Shanghai Jinpan Biotech Co Ltd. We are authorized agent of Boykyo Pharmaceutical Co Ltd. Boykyo Pharmaceutical Co Ltd is one Japanese Company. Boykyo Pharmaceutical Co Ltd can produce three main products which are good alternative products of Streck,biomatrica and Genotek.

We have three main product:

1、 Boykyo Cell-Free DNA Blood Collection tube :Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of streck Cell-Free DNA BCT 218962. We have the same Lower prices for the same quality.

卜一口无创基因采血管蓝头

2、 Boykyo Saliva Collection Device:Samples collected in Boykyo Saliva Collection Device are stable for up to 36 months at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Biomatrica DNAgard® Saliva HT and Oragene•DNA OG 500 of genotek. We have the same Lower prices for the same quality.

 邮件唾液采集器  唾液采集器2 唾液采集器3 唾液采集器4

3、 Whole-Blood DNA Blood Collection tube :Samples collected in Whole-Blood DNA Blood Collection tube  for up to 30 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Streck Cyto-Chex® BCT  213386. We have the same Lower prices for the same quality.

We are fingding distributor of the three main products in your country and We are looking forward to being to cooperate with you.

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

Website of Boykyo: http://www.boykyo.com/

Chinese wbebsite of Boykyo : http://www.boykyo.com.cn

Wbebsite of Shanghai Jinpan Biotech Co Ltd : http://www.jinpanbio.cn

Picture of Cell-Free DNA Blood Collection tube

卜一口无创基因采血管蓝头

Picture of  Boykyo Saliva Collection Device

邮件唾液采集器

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address:No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

Streck Cell-Free DNA BCT 218961 alternative products of Streck

Streck Cell-Free DNA BCT 218961 alternative products of Streck

6-Tube Pack (10 mL) 218961
100-Tube Box (10 mL) 218962
1000-Tube Box (10 mL) 218992
6-Tube Pack (2 mL) 230242
100-Tube Box (2 mL) 230243
Cell-Free DNA BCT CE is For Export Only. Not for sale in the U.S.
6-Tube Pack (10 mL) 218996
100-Tube Box (10 mL) 218997
1000-Tube Box (10 mL) 230244
6-Tube Pack (2 mL) 230250
100-Tube Box (2 mL) 230251

Cell-Free DNA BCT Blood Collection Tube

Streck Cell-Free DNA BCT 218962 10 mL  alternative products of Streck

Now available in two sizes: 10 mL and 2 mL

Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Cell-Free DNA BCT is for Research Use Only. Not for use in diagnostic procedures in the U.S.

Cell-Free DNA BCT CE is for Export Only. Not for sale in the U.S.

Paper: Stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping

Click here to see patents that may be applicable to this product.

Resources
Catalog Numbers
Cell-Free DNA BCT Catalog Number
Cell-Free DNA BCT is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack (10 mL) 218961
100-Tube Box (10 mL) 218962
1000-Tube Box (10 mL) 218992
6-Tube Pack (2 mL) 230242
100-Tube Box (2 mL) 230243
Cell-Free DNA BCT CE is For Export Only. Not for sale in the U.S.
6-Tube Pack (10 mL) 218996
100-Tube Box (10 mL) 218997
1000-Tube Box (10 mL) 230244
6-Tube Pack (2 mL) 230250
100-Tube Box (2 mL) 230251

 

We are one company from china. Our Company name is Shanghai Jinpan Biotech Co Ltd. We are authorized agent of Boykyo Pharmaceutical Co Ltd. Boykyo Pharmaceutical Co Ltd is one Japanese Company. Boykyo Pharmaceutical Co Ltd can produce three main products which are good alternative products of Streck,biomatrica and Genotek.

We have three main product:

1、 Boykyo Cell-Free DNA Blood Collection tube :Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of streck Cell-Free DNA BCT 218962. We have the same Lower prices for the same quality.

卜一口无创基因采血管蓝头

 

 

2、 Boykyo Saliva Collection Device:Samples collected in Boykyo Saliva Collection Device are stable for up to 36 months at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Biomatrica DNAgard® Saliva HT and Oragene•DNA OG 500 of genotek. We have the same Lower prices for the same quality.

 邮件唾液采集器  唾液采集器2 唾液采集器3 唾液采集器4

3、 Whole-Blood DNA Blood Collection tube :Samples collected in Whole-Blood DNA Blood Collection tube  for up to 30 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Streck Cyto-Chex® BCT  213386. We have the same Lower prices for the same quality.

 

We are fingding distributor of the three main products in your country and We are looking forward to being to cooperate with you.

 

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

Website of Boykyo: http://www.boykyo.com/

Chinese wbebsite of Boykyo : http://www.boykyo.com.cn

Wbebsite of Shanghai Jinpan Biotech Co Ltd : http://www.jinpanbio.cn

Picture of Cell-Free DNA Blood Collection tube

卜一口无创基因采血管蓝头

Picture of  Boykyo Saliva Collection Device

邮件唾液采集器

 

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

Streck Cell-Free DNA BCT 218962 alternative products of Streck

Cell-Free DNA BCT Blood Collection Tube

Streck Cell-Free DNA BCT 218962 10 mL  alternative products of Streck

Now available in two sizes: 10 mL and 2 mL

Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Cell-Free DNA BCT is for Research Use Only. Not for use in diagnostic procedures in the U.S.

Cell-Free DNA BCT CE is for Export Only. Not for sale in the U.S.

Paper: Stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping

Click here to see patents that may be applicable to this product.

Resources
Catalog Numbers
Cell-Free DNA BCT Catalog Number
Cell-Free DNA BCT is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack (10 mL) 218961
100-Tube Box (10 mL) 218962
1000-Tube Box (10 mL) 218992
6-Tube Pack (2 mL) 230242
100-Tube Box (2 mL) 230243
Cell-Free DNA BCT CE is For Export Only. Not for sale in the U.S.
6-Tube Pack (10 mL) 218996
100-Tube Box (10 mL) 218997
1000-Tube Box (10 mL) 230244
6-Tube Pack (2 mL) 230250
100-Tube Box (2 mL) 230251

 

We are one company from china. Our Company name is Shanghai Jinpan Biotech Co Ltd. We are authorized agent of Boykyo Pharmaceutical Co Ltd. Boykyo Pharmaceutical Co Ltd is one Japanese Company. Boykyo Pharmaceutical Co Ltd can produce three main products which are good alternative products of Streck,biomatrica and Genotek.

We have three main product:

1、 Boykyo Cell-Free DNA Blood Collection tube :Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of streck Cell-Free DNA BCT 218962. We have the same Lower prices for the same quality.

卜一口无创基因采血管蓝头

 

 

2、 Boykyo Saliva Collection Device:Samples collected in Boykyo Saliva Collection Device are stable for up to 36 months at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Biomatrica DNAgard® Saliva HT and Oragene•DNA OG 500 of genotek. We have the same Lower prices for the same quality.

 邮件唾液采集器  唾液采集器2 唾液采集器3 唾液采集器4

3、 Whole-Blood DNA Blood Collection tube :Samples collected in Whole-Blood DNA Blood Collection tube  for up to 30 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage. This is good alternative products of Streck Cyto-Chex® BCT  213386. We have the same Lower prices for the same quality.

 

We are fingding distributor of the three main products in your country and We are looking forward to being to cooperate with you.

 

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

Website of Boykyo: http://www.boykyo.com/

Chinese wbebsite of Boykyo : http://www.boykyo.com.cn

Wbebsite of Shanghai Jinpan Biotech Co Ltd : http://www.jinpanbio.cn

Picture of Cell-Free DNA Blood Collection tube

卜一口无创基因采血管蓝头

Picture of  Boykyo Saliva Collection Device

邮件唾液采集器

 

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ:  3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New  Area,Shanghai, China
Website: www.jinpanbio.com  www.jinpanbio.cn

 

Boykyo Cell-Free DNA Blood Collection Tube

Boykyo Cell-Free DNA BCT

Japanese website: www.boykyo.com
Chinese website: www.boykyo.com.cn

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New
Area,Shanghai, China
Website: www.jinpanbio.com www.jinpanbio.cn

Blood Collection Tube

Boykyo Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Boykyo Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Boykyo Cell-Free DNA BCT Catalog Number
Cell-Free DNA BCT is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack 691012
100-Tube Box 691013

INSTRUCTIONS FOR USE
INTENDED USE

Boykyo Cell-Free DNA BCT? is a direct draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plasma DNA. This device also stabilizes and preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic use. The product is For Research Use Only. Not for use in diagnostic procedures.

SUMMARY AND PRINCIPLES

Accurate analysis of cf-DNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular genomic DNA. Additionally, degradation of cf-DNA due to nuclease activity can be problematic.

The formaldehyde-free preservative reagent contained in Cell-Free DNA BCT stabilizes nucleated blood cells, preventing the release of cellular genomic DNA, and inhibits nuclease mediated degradation of cf-DNA, contributing to the overall stabilization of cf-DNA3 . Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage4.

The formaldehyde-free preservative reagent contained in Cell-Free DNA BCT stabilizes circulating epithelial cells (tumor cells) in whole blood for up to 4 days at temperatures between 15 °C-30 °C5.

REAGENTS
Cell-Free DNA BCT contains the anticoagulant K3EDTA and a cell preservative in a liquid medium.

PRECAUTIONS

1. For Research Use Only. Not for use in diagnostic procedures.
2. Do not freeze specimens collected in Cell-Free DNA BCT as breakage could result.
3. Do not use tubes after expiration date.
4. Do not use tubes for collection of materials to be injected into patients.
5. Product is intended for use as supplied. Do not dilute or add other components to Cell-Free DNA BCT.
6. Overfilling or underfilling of tubes will result in an incorrect blood-to-additive ratio and may lead to incorrect analytic results or poor product performance.

CAUTION
a. Glass has the potential for breakage; precautionary measures should be taken during handling.
b. All biological specimens and materials coming in contact with them are considered biohazards and should be treated as if capable of transmitting infection. Dispose of in accordance with federal, state and local regulations. Avoid contact with skin and mucous membranes.
c. Product should be disposed with infectious medical waste.
d. Remove and reinsert stopper by either gently rocking the stopper from side to side or by grasping with a simultaneous twisting and pulling action. A “thumb roll” procedure for stopper removal is NOT recommended as tube breakage and injury may result.
7. SDS can be obtained at www.boykyo.com or by calling +86-021-50837765.

STORAGE AND STABILITY

1. When stored at 18 °C-30 °Cunused Cell-Free DNA BCT is stable through expiration date.
2. Do not freeze unfilled Cell-Free DNA BCT. Proper insulation may be required for shipment during extreme temperature conditions.
3. Blood samples collected in Cell-Free DNA BCT for cf-DNA analysis are stable for 14 days when stored between 6 °C-37 °C.
4. Blood samples collected in Cell-Free DNA BCT for genomic DNA analysis are stable for 14 days when stored between 6 °C-37 °C.
5. Blood samples collected in Cell-Free DNA BCT for circulating epithelial cells (tumor cells) are stable for 4 days when stored between 15 °C-30 °C.
INDICATIONS OF PRODUCT DETERIORATION

1. Cloudiness or precipitate visible in reagent of unused tube.
2. If indications of product deterioration occur, contact Boykyo Technical Services at +86-021-50837765 or service@boykyo.com.

INSTRUCTIONS FOR USE

1. Collect specimen by venipuncture according to CLSI H3-A66.Prevention of Backflow – Since Cell-Free DNA BCT contains chemical additives, it is important to avoid possible backflow from the tube. To guard against backflow, observe the following precautions:

a. Keep patient’s arm in the downward position during the collection procedure.
b. Hold the tube with the stopper in the uppermost position so that the tube contents do not touch the stopper or the end of the needle during sample collection.
c. Release tourniquet once blood starts to flow in the tube, or within 2 minutes of application.

2. Follow recommendations for order of draw outlined in CLSI H3-A66. Cell-Free DNA BCT can be drawn after the EDTA tube and before the Glycolytic inhibitor tube.
3. Fill tube completely.
4. Remove tube from adapter and immediately mix by gentle inversion 8 to 10 times. Inadequate or delayed mixing may result in inaccurate test results. One inversion is a complete turn of the wrist, 180 degrees, and back per the figure below:
5. After collection, transport and store tubes within the recommended temperature range

DNA EXTRACTION

Extraction of cell-free plasma DNA and cellular genomic DNA can be accomplished using most commercially available kits that include a Proteinase K treatment step.
Cell-Free Plasma DNA
Step 1. To separate plasma, centrifuge whole blood at 300 x g for 20 minutes at room temperature.
Step 2. Remove the upper plasma layer and transfer to a fresh tube.
Step 3. Centrifuge the plasma at 5000 x g for 10 minutes.
Step 4. Isolate cell-free DNA per kit manufacturer instructions.

For optimal results, include a Proteinase K treatment step (≥30 mAU/mL digest) at 60 ℃ in the presence of chaotropic salts for 1 hour when extracting cell-free DNA.
Cellular Genomic DNA
Step 1. To separate the white blood cells, either lyse the red blood cells and wash, or centrifuge whole blood and collect the buffy coat layer.
Step 2. Isolate genomic DNA per kit manufacturer instructions. or optimal results, include a Proteinase K treatment step (≥ 30 mAU/ml digest) at 60 ℃in the presence of chaotropic salts for 2 hours when extracting cellular genomic DNA.

Note:
1. Cell-Free DNA BCT does not dilute blood samples; therefore, no dilution factor correction is necessary.
2. As in the case with most clinical laboratory specimens, hemolysis, icterus and lipemia may affect the results obtained on blood samples preserved with Cell-Free DNA BCT.

LIMITATIONS
1. For single use only.
2. Samples drawn in other anticoagulants or preservatives may cause coagulation in Cell-Free DNA BCT.
3. The use of butterfly collection needles for venipuncture is not recommended with the 10ml Cell-Free DNA BCT.

REFERENCES

Can be found online at www.boykyo.com.

Boykyo Cell-Free DNA BCT

Boykyo Cell-Free DNA BCT

Japanese website: www.boykyo.com
Chinese website: www.boykyo.com.cn

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Address: Room 101, Building 38, No.669, Dongjing Rord, Pudong New
Area,Shanghai, China
Website: www.jinpanbio.com www.jinpanbio.cn

Blood Collection Tube

Boykyo Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Boykyo Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Boykyo Cell-Free DNA BCT Catalog Number
Cell-Free DNA BCT is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack 691012
100-Tube Box 691013

INSTRUCTIONS FOR USE
INTENDED USE

Boykyo Cell-Free DNA BCT? is a direct draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plasma DNA. This device also stabilizes and preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic use. The product is For Research Use Only. Not for use in diagnostic procedures.

SUMMARY AND PRINCIPLES

Accurate analysis of cf-DNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular genomic DNA. Additionally, degradation of cf-DNA due to nuclease activity can be problematic.

The formaldehyde-free preservative reagent contained in Cell-Free DNA BCT stabilizes nucleated blood cells, preventing the release of cellular genomic DNA, and inhibits nuclease mediated degradation of cf-DNA, contributing to the overall stabilization of cf-DNA3 . Samples collected in Cell-Free DNA BCT are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage4.

The formaldehyde-free preservative reagent contained in Cell-Free DNA BCT stabilizes circulating epithelial cells (tumor cells) in whole blood for up to 4 days at temperatures between 15 °C-30 °C5.

REAGENTS
Cell-Free DNA BCT contains the anticoagulant K3EDTA and a cell preservative in a liquid medium.

PRECAUTIONS

1. For Research Use Only. Not for use in diagnostic procedures.
2. Do not freeze specimens collected in Cell-Free DNA BCT as breakage could result.
3. Do not use tubes after expiration date.
4. Do not use tubes for collection of materials to be injected into patients.
5. Product is intended for use as supplied. Do not dilute or add other components to Cell-Free DNA BCT.
6. Overfilling or underfilling of tubes will result in an incorrect blood-to-additive ratio and may lead to incorrect analytic results or poor product performance.

CAUTION
a. Glass has the potential for breakage; precautionary measures should be taken during handling.
b. All biological specimens and materials coming in contact with them are considered biohazards and should be treated as if capable of transmitting infection. Dispose of in accordance with federal, state and local regulations. Avoid contact with skin and mucous membranes.
c. Product should be disposed with infectious medical waste.
d. Remove and reinsert stopper by either gently rocking the stopper from side to side or by grasping with a simultaneous twisting and pulling action. A “thumb roll” procedure for stopper removal is NOT recommended as tube breakage and injury may result.
7. SDS can be obtained at www.boykyo.com or by calling +86-021-50837765.

STORAGE AND STABILITY

1. When stored at 18 °C-30 °Cunused Cell-Free DNA BCT is stable through expiration date.
2. Do not freeze unfilled Cell-Free DNA BCT. Proper insulation may be required for shipment during extreme temperature conditions.
3. Blood samples collected in Cell-Free DNA BCT for cf-DNA analysis are stable for 14 days when stored between 6 °C-37 °C.
4. Blood samples collected in Cell-Free DNA BCT for genomic DNA analysis are stable for 14 days when stored between 6 °C-37 °C.
5. Blood samples collected in Cell-Free DNA BCT for circulating epithelial cells (tumor cells) are stable for 4 days when stored between 15 °C-30 °C.
INDICATIONS OF PRODUCT DETERIORATION

1. Cloudiness or precipitate visible in reagent of unused tube.
2. If indications of product deterioration occur, contact Boykyo Technical Services at +86-021-50837765 or service@boykyo.com.

INSTRUCTIONS FOR USE

1. Collect specimen by venipuncture according to CLSI H3-A66.Prevention of Backflow – Since Cell-Free DNA BCT contains chemical additives, it is important to avoid possible backflow from the tube. To guard against backflow, observe the following precautions:

a. Keep patient’s arm in the downward position during the collection procedure.
b. Hold the tube with the stopper in the uppermost position so that the tube contents do not touch the stopper or the end of the needle during sample collection.
c. Release tourniquet once blood starts to flow in the tube, or within 2 minutes of application.

2. Follow recommendations for order of draw outlined in CLSI H3-A66. Cell-Free DNA BCT can be drawn after the EDTA tube and before the Glycolytic inhibitor tube.
3. Fill tube completely.
4. Remove tube from adapter and immediately mix by gentle inversion 8 to 10 times. Inadequate or delayed mixing may result in inaccurate test results. One inversion is a complete turn of the wrist, 180 degrees, and back per the figure below:
5. After collection, transport and store tubes within the recommended temperature range

DNA EXTRACTION

Extraction of cell-free plasma DNA and cellular genomic DNA can be accomplished using most commercially available kits that include a Proteinase K treatment step.
Cell-Free Plasma DNA
Step 1. To separate plasma, centrifuge whole blood at 300 x g for 20 minutes at room temperature.
Step 2. Remove the upper plasma layer and transfer to a fresh tube.
Step 3. Centrifuge the plasma at 5000 x g for 10 minutes.
Step 4. Isolate cell-free DNA per kit manufacturer instructions.

For optimal results, include a Proteinase K treatment step (≥30 mAU/mL digest) at 60 ℃ in the presence of chaotropic salts for 1 hour when extracting cell-free DNA.
Cellular Genomic DNA
Step 1. To separate the white blood cells, either lyse the red blood cells and wash, or centrifuge whole blood and collect the buffy coat layer.
Step 2. Isolate genomic DNA per kit manufacturer instructions. or optimal results, include a Proteinase K treatment step (≥ 30 mAU/ml digest) at 60 ℃in the presence of chaotropic salts for 2 hours when extracting cellular genomic DNA.

Note:
1. Cell-Free DNA BCT does not dilute blood samples; therefore, no dilution factor correction is necessary.
2. As in the case with most clinical laboratory specimens, hemolysis, icterus and lipemia may affect the results obtained on blood samples preserved with Cell-Free DNA BCT.

LIMITATIONS
1. For single use only.
2. Samples drawn in other anticoagulants or preservatives may cause coagulation in Cell-Free DNA BCT.
3. The use of butterfly collection needles for venipuncture is not recommended with the 10ml Cell-Free DNA BCT.

REFERENCES

Can be found online at www.boykyo.com.

Streck Cell-Free RNA BCT Blood Collection Tube

Cell-Free RNA BCT®Please note: calibrators, linearity products, PVS, and thermometers are not available as a sample.

Cell-Free RNA BCT®  Blood Collection Tube

Cell-Free RNA BCT is  a blood collection tube with a patented preservative stabilizing cell-free RNA in plasma and prevents the release of non-target background RNA from blood cells during sample processing and storage.

Cell-Free RNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free RNA is stable for up to 7 days at room temperature, allowing convenient sample collection, transport and storage.

For Research Use Only. Not for use in diagnostic procedures.

Paper: Stabilization of Cell-Free RNA in blood samples

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Streck Cyto-Chex® BCTCE Blood Collection Tube

Cyto-Chex® BCTCE

Blood Collection Tube

Cyto-Chex BCT is a blood collection tube for the preservation of whole blood specimens for immunophenotyping by flow cytometry. The preservative contained in Cyto-Chex BCT maintains the integrity of the white blood cell cluster of differentiation (CD) markers at room temperature for convenient transport and storage.

Cyto-Chex BCT preserves peripheral blood samples’ qualitative and quantitative leukocyte subset characteristics and is FDA 510(k) cleared for consistent recovery of HIV associated lymphocyte subsets for up to 14 days.

Available in 5.0 mL and 2.0 mL direct draw tubes.

Click here to see patents that may be applicable to this product.

Cyto-Chex BCT Mixing Instructions »

Cell-Free DNA BCT® Blood Collection Tube

Cell-Free DNA BCT®

Blood Collection Tube Now available in two sizes: 10 mL and 2 mL Cell-Free DNA BCT is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development. Cell-Free DNA BCT reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage. Cell-Free DNA BCT is for Research Use Only. Not for use in diagnostic procedures in the U.S. Cell-Free DNA BCT CE is for Export Only. Not to be sold in the U.S. Paper: Stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping Click here to see patents that may be applicable to this product.